Abstrac
Respiratory infections are the most common infectious diseases in humans worldwide and a leading cause of death in children less than 5 years of age.1–3 In the United States of America, acute viral respiratory infections (ARIs) are a significant cause of morbidity and hospitalization in children.4,5 Identification of previouslyunknown respiratory pathogens may lead to the development of new therapies and vaccines to treat or prevent ARIs. Recently, a sensitive and highly multiplexed PCR platform, MassTag-PCR, was designed to address the limitations of conventional multiplex PCR.6 MassTag-PCR simultaneously detects 20–30
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http://www.ziddu.com/download/15072425/jurnalJournalofClinicalVirology.pdf.html
- Background: Respiratory infections are the most common infectious diseases in humans worldwide and are a leading cause of death in children less than 5 years of age.
- Objectives: Identify candidate pathogens in pediatric patients with unexplained respiratory disease.
- Study design: Forty-four nasopharyngeal washes collected during the 2004–2005 winter season from pediatric patients with respiratory illnesses that tested negative for 7 common respiratory pathogens by culture and direct immunofluorescence assays were analyzed by MassTag-PCR. To distinguish human enteroviruses (HEV) and rhinoviruses (HRV), samples positive for picornaviruses were further characterized by sequence analysis.
- Results: Candidate pathogens were detected by MassTag PCR in 27 of the 44 (61%) specimens that previouslywere rated negative. Sixteen of these 27 specimens (59%) contained picornaviruses; of these 9 (57%) contained RNA of a recently discovered clade of rhinoviruses. Bocaviruseswere detected in three patients by RT-PCR.
- Conclusions: Our study confirms that multiplex MassTag-PCR enhances the detection of pathogens in clinical specimens, and shows that previously unrecognized rhinoviruses, that potentially form a species HRV-C, may cause a significant amount of pediatric respiratory disease.
Respiratory infections are the most common infectious diseases in humans worldwide and a leading cause of death in children less than 5 years of age.1–3 In the United States of America, acute viral respiratory infections (ARIs) are a significant cause of morbidity and hospitalization in children.4,5 Identification of previouslyunknown respiratory pathogens may lead to the development of new therapies and vaccines to treat or prevent ARIs. Recently, a sensitive and highly multiplexed PCR platform, MassTag-PCR, was designed to address the limitations of conventional multiplex PCR.6 MassTag-PCR simultaneously detects 20–30
Full Link Download
http://www.ziddu.com/download/15072425/jurnalJournalofClinicalVirology.pdf.html
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